Growth Dynamics Of E. Coli In Varying Concentrations Of Nutrient Broth :: essays research papers

Growth Dynamics of E. coli in Varying Concentrations of Nutrient Broths, pH, and in the Presence of an Antibiotic Abstract The purpose in this experiment of growth dynamics of E. coli in varying media was to determine which media produces the maximum number of cells per unit time. First a control was established for E. coli in a 1.0x nutrient broth. This was used to compare the growth in the experimental media of 0.5x and 2.0x, nutrient broths; nutrient broths with an additional 5.0mM of glucose and another with 5.0mM lactose; nutrient broths of varying pH levels: 6.0, 7.0, and 8.0; and finally a nutrient broth in the presence of the drug/antibiotic chloramphenicol. A variety of OD readings were taken and calculations made to determine the number of cells present after a given time. Then two graphs were plotted, Number of cells per unit volume versus Time in minutes and Log of the number of cells per unit volume versus Time growth curve. The final cell concentration for the control was 619,500 cells/mL. Four media, after calculations, produced fewer cells than that of the control, these were: Chloramphenicol producing 89,3 01 cells/ml; glucose producing 411,951 cells/mL; lactose producing 477,441 cells/mL and finally pH 6.0 producing 579,557cells/mL. The remaining four media, after calculations, produced cell counts greater than the control: 2X with 1,087,009 cells/mL; 0.5X with 2,205,026 cells/mL; pH 8 with 3,583,750 cells/mL and finally pH 7.0 with 8,090,325 cells/mL. From these results the conclusion can be made that the environment is a controlling factor in the growth dynamics of E. coli. This was found through the regulation of pH and nutrient concentrations. In the presence of the drug/antibiotic, chloramphenicol, cell growth was minimal. Introduction E. coli grows and divides through asexual reproduction. Growth will continue until all nutrients are depleted and the wastes rise to a toxic level. This is demonstrated by the Log of the number of cells per unit volume versus Time growth curve. This growth curve consists of four phases: Lag, Exponential, Stationary, and finally Death. During the Lag phase there is little increase in the number of cells. Rather, during this phase cells increase in size by transporting nutrients inside the cell from the medium preparing for reproduction and synthesizing DNA and various enzymes needed for cell division. In the Exponential phase, also called the log growth phase, bacterial cell division begins. The number of cells increases as an exponential function of time. The third phase, Stationary, is where the culture has reached a phase during which there is no net increase in the number of cells. During the

Factors Affecting Essay

Few markets avoid change, which involves new products or new ways of making existing products. Some industries are more likely to undergo substantial changes brought about by new technologies and new adaptations of existing technologies. The IT, computer and mobile phone markets are experiencing more innovation than most. How do businesses benefit from introducing radical new products and what factors influence the range and pace of innovation? Spending on research and development (R&D) is growing globally and in most industrial sectors. The benefits to a country of encouraging R&D spending include: †¢creation of high-tech jobs †¢creation of high-added-value products that may then be manufactured in that country †¢prestige – a country being linked to scientific and technological breakthroughs †¢Attraction of investment by multinational corporations. Several factors may influence the level of research and development (R&D) and innovation by a business: †¢The nature of the industry. Rapidly changing technologies – and consumer expectations – in pharmaceutical products, defense, computer and software products and motor vehicles lead to the need for substantial investment in R&D by leading firms. Other businesses, such as hotels and hairdressing, would need to spend much less as the scope for innovation is more limited. †¢The R&D and innovation spending plans of competitors. In most markets, it is essential to innovate as much as or more than competitors if market share and technical leadership are to be maintained. However, a monopoly may limit R&D spending if it believes that the risk of a more technically advanced competitor entering the market is limited. On the other hand, profits from a monopoly could be used to finance research into innovative products if the risk of competitor entry into the industry is high. †¢Business expectations. If business managers are optimistic about the future state of the economy and the rate of economic growth and consumer demand, then they are more likely to agree to substantial budgets for R&D and aim to introduce more innovative products. †¢The risk profile or culture of the business. The attitude of the management to risk and whether shareholders are prepared to invest for the long term will have a significant effect on the sums that businesses can inject into R&D programmes. ‘Short-termism’ is an accusation made towards many major UK financial institutions and the need to satisfy these investors could discourage managers from investing in R&D. Government policy towards grants to businesses and universities for R&D programmes and the range and scope of tax allowances for such expenditure will influence decisions by businesses. †¢Finance is needed for effective R&D. In many firms this may be limited and will restrict the number of new innovations that could be made. Reference: http://classof1.com/homework-help/operations-management-homework-help View as multi-pages

Abigail Williams Analyzation

Williams is a cruel, vengeful and power hungry person. She has the ability to manipulate almost anyone she wants, whenever and wherever. She uses this ability to gain power and deem people witches who don't have any real proof against them. This puts her in a position where she can Just do almost anything she wants to, like eliminating people that she doesn't want to see anymore. Abigail Williams is really just one power hungry, vengeful person. Abigail had been one of the original girls that started the whole witchcraft ordeal y practicing voodoo rituals in the woods outside of her town.She later then blamed the whole event on the slave of Reverend Paris saying â€Å"Now look you. All of you. We danced. And Tuba conjured Ruth Putnam dead sisters. † The people she told believed her as slaves were of lower class then Abigail, and that Just happened to be how it worked then. Abigail also was in an affair with John Proctor, which after Elizabeth found out about it, who is John's wife, Abigail was fired from her position as a servant to the Proctors.Abigail went on to accuse people of the town of witchcraft. This allowed her to rise to power and become a member of the court. Abigail sought vengeance on the people that witnessed her in the woods with Tuba as well. She pin pointed those people and decided she would accuse them of witchcraft In any way she could. For instance, she accused Elizabeth of witchcraft because she had a doll with a needle In Its stomach, saying she was using voodoo to hurt Abigail.Even though Abigail set the whole thing up and punctured herself with a needle. All these situations show how Abigail nature Is to be cruel, and how she manipulates people's views and emotions. Abigail also became a huge power fugue throughout the act. She rose to power after accusing people for witchcraft. She made up fake stories and false evidence to get people to go to trial. For Instance when she accused Elizabeth proctor of witchcraft, Abigail said she was stabbed by Elizabethan spirit.When the authorities searched the Proctors house, they found a doll with a needle In Its stomach. This was enough â€Å"proof' they needed to take her away. Abigail got away with a lot of things throughout act 1 . She used peoples emotions to get what she wanted and didn't have any sympathy for anyone she came across. She ended up In a position where she can Just do almost anything she wants to, Like eliminating people that she doesn't want to see anymore. Ball Williams Is really Just one power hungry, vengeful person.Abigail Williams Analyzing By shinning pin pointed those people and decided she would accuse them of witchcraft in any OLL with a needle in its stomach, saying she was using voodoo to hurt Abigail. Even situations show how Abigail nature is to be cruel, and how she manipulates people's false evidence to get people to go to trial. For instance when she accused Elizabeth authorities searched the Proctors house, they found a doll with a needle in its stomach. This was enough â€Å"proof† they needed to take her away. She ended up in a position where she can Just do almost anything she wants to, like

Good Will Hunting: Movie Response

Good Will Hunting Movie Response Name: answer each questions with 1 paragraph (6 sentences) Please 1 . What is Sean Maguire's (Robin Williams) Psychological Specialization? How do you know this? What parts of the movie lead you to this conclusion? Sean Maguire specializes in talking with people who are incredibly defensive and unwilling to open up about many aspects of life. Sean could be considered a Counseling Psychologist. This becomes quite evident as their time together goes on throughout the film.Will is completely unwilling to open up in the first few sessions they have together, and shows a great deal of defensiveness. He dodges and redirects questions, and Sean goes along with it for a while. Finally, Sean actually challenges Will's weak defense mechanisms, and after a few unproductive sessions, Will begins to open up. 2. What contemporary school of psychological thought does Sean Maguire belong to? How do you know this? What parts of the movie lead you to this conclusion? 3 .What have you gained from watching the movie that you can translate into your own life? I have learned that we are all eventually trying to find our purpose in life. Some take longer than others. However, a little help and a push in the right direction helps speed up the process, unfortunately millions of people do not get that opportunity. Sadly, many spend their lives doing something that is nowhere close to what they are really meant to be doing. Unfortunately, in today's world, circumstances determine what and how you eve to live your life.We spend so much time thinking what others perceive us as that we eventually lose track of who we really are. A classroom can teach you many things life cannot. So long as you are willing to learn from everything and everyone around you. We all need genuine friends and catalysts to help us find our journey. How we respond to their advice helps speed up the process. We all need to find someone that makes us tick. In addition, never let go of t hem. It helps if they are of the opposite sex – only if that is your preference.

Applying Theories to the real world cases Case Study

Applying Theories to the real world cases - Case Study Example Criminal behavior is largely seen to be learned behavior. It is also influenced by peer pressure, institutional breakdown, social environment and financial difficulties. It is through appropriate measures that the vice will be addressed appropriately. Implementing stringent punitive measures coupled with proper policy frameworks and legislations will go a long way in addressing the warring problems associated with criminality. Criminality offends the standards and values that characterize a civilized society that believes in the right to life. in his study on criminology, Abrahams (2015) observes that men consitute the highest percentage of prisoners in many nations around the world. According to the International Centre for Prison Studies, the global percentage of women in prisons constitutes about 4.3 percent. Abrahams (2015) alleges that men are likely to engage in criminal activities compared to women. While women engage in crminal activities, the gap between the two genders requires an assessment on the factors that contribute to higher criminal behavior in men. Men engage in minor offences, white collar crimes, murder and riots, among others. some of the contributors highlighted by the author include lack of education, delinguent behaviors, street gangs, peer influences, and financial difficulties, among others. Male criminality has for a long period overshadowed female criminality. However, the author argues that female criminality has been on the rise. The incidences and significance of female criminality are mini mal with some going unnoticed. Nowadays, female crime patterns have changed with offenses such as drug trafficking, white-collar crimes and violent robbery reported among women. Male criminals operate in well-connected groups that have deep connection with the authorities, thus making it hard for them to be combated. Their actions threaten the social order in a society. In Britain, for instance, Abrahams

E-goverment in angola Dissertation Example | Topics and Well Written Essays - 9500 words

E-goverment in angola - Dissertation Example One aspect of change is e-governance. Though existing literature has outlined the possibility of e-governance spiraling economic lives of citizens, little has been done to unravel understanding of the prospects, challenges, and practices of e-governance in improving literacy levels, championing the values of democracy, increasing economic transactions, and improving government administrative functions. This report seeks to explore the prospects of E-governance in Angola, assess the challenges facing implementation of e-governance, and ultimately establish best practices that have effectively promoted the governance in Angola. A survey will help gather qualitative data to be analyzed using descriptive statistics. The target population will be government departments, businesses, citizens, and government employees. This study will help guide the government of Angola to determine how e-governance has and will help in service delivery, know sections of the population not reached or utiliz ing the services and promote effective inter-ministerial or departmental communication in the nation of Angola. Table of Contents Abstract 2 Table of Contents 3 1.0 Introduction 5 2.0 Literature Review 6 2.2.1Government and Governance 7 2.2.2 E-Government and E-Governance 8 2.2.3 Components of E-Government 8 2.2.4 E-governance 9 2.3 E-Government in Angola 10 2.3.1 Overview of Angola 10 2.3.2 Demography 11 2.4.3 Telecommunications Infrastructure 14 2.4.4 Human Development (Human Index in Angola) 16 2.6 Opportunity 18 2.6.1 Quality of Public Services to Citizen 18 2.6.3 Encourage Use of ICT Africa 20 2.7.1 Angola: Country Profile 21 2.7.2 Demography 22 2.7.3 Economy 22 2.7.4 Governance 24 2.7.5 Description 26 2.7.6 Specific Objectives 27 3.1 Sample size 30 1.0 Introduction This study intends to analyze the role of e-governance in fostering democracy in Africa by critically assessing its contribution on the Angolan governance and how these aspects have contributed to effective service delivery and improved the standards of living of Angolan people. It outlines how to subdue the challenges of instituting e-governance and further inform the knowledge bank of the Angolan government on the current and new challenges likely to be faced in implementation. 1.1 Research Question To what extent has e-governance influenced democracy and good governance in Africa? 1.2 Objectives: 1. Explore the prospects of e-governance in Africa. 2. Assess the challenges facing implementation of e-governance in Africa. 3. Establish best practices that have effectively promoted democracy and good governance in Africa. 2.0 Literature Review 2. 1 Introduction In 1990s, the IMF imposed structural adjustment programs meant to ease Africa’s economic hardships and act as an avenue for developing good use of public resources and promotion of accountability by governments to citizens. In order to meet the donor demands and increased pressure by citizens to have their governments perform, Afr ican countries gradually began to appreciate the use of internet, PCs, mobile phones, and other gadgets to deliver government services online. E-government is taunted as the most efficient way of streamlining public sector reforms and citizen involvement in administration and governance of public affairs. Though the process of e-governance is highly admired, there are many challenges, facing its implementation.

The Role of Information Security Policy Essay Example | Topics and Well Written Essays - 1000 words

The Role of Information Security Policy - Essay Example Regardless of all these, the essence for a security policy on information systems is unquestionable. These security policies and standards are of use to inform employees of firm their duties for protecting and safeguarding information systems of their organization. The policies state the mechanisms under which organizations can carry out these responsibilities. They also give an organization the baseline to get, configure and appraisal information systems for compliance with the policy in operation. The tools of information security system without its policy, it limits its usefulness. Policies and standards are essential in distributed computing location as a means of putting up security discipline for a big and different population of users that is reachable through formal auditing and communications. This strategy is of great importance when dealing with temporal personnel or those under contracts. Policies must show the accepted ways of an organization but also take full advantage of all methods for effecting behavior and spreading of information within a distributed computing location (James et al, 2012). Policies are of more importance in distributed computing locations than a centralized one due to the increased problems of restraining activities coming from a remote location. These policies must therefore be complete and stated clearly to minimize the number of instructions and explanations that the organization requires to handle so that they can be sure there is understanding. Here, they should have identifiers and description generally for business functions and units so that they can surpass changes of the organization (James et al, 2012). Employees play a major role in maintaining information system security. Their main role here is to study and have a close relationship with the IT section so that they can indulge when there is security incident. It is the responsibility of every employee to